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chondroitinase abc enzyme  (AMS Biotechnology)


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    AMS Biotechnology chondroitinase abc enzyme
    ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with <t>chondroitinase</t> <t>ABC</t> <t>(chABC)</t> at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.
    Chondroitinase Abc Enzyme, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A neuroprotective tetrapeptide for treatment of acute traumatic brain injury"

    Article Title: A neuroprotective tetrapeptide for treatment of acute traumatic brain injury

    Journal: EMBO Molecular Medicine

    doi: 10.1038/s44321-025-00312-5

    ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with chondroitinase ABC (chABC) at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.
    Figure Legend Snippet: ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with chondroitinase ABC (chABC) at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.

    Techniques Used: Fluorescence, Binding Assay, Incubation, FP Assay, Labeling, Purification, Isolation, Cell Culture



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    ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with <t>chondroitinase</t> <t>ABC</t> <t>(chABC)</t> at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.
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    ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with <t>chondroitinase</t> <t>ABC</t> <t>(chABC)</t> at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.
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    Aggrecanase activity of ADAMTS9 MDTCS. Increasing concentrations of ADAMTS9 MDTCS (0.025–25 nM) or 5 nM full-length ADAMTS5 were incubated with bovine aggrecan (400 nM). Digests were stopped after two or 24 h and cleavage fragments deglycosylated and subjected to SDS-PAGE. Bands were detected using anti-aggrecan ARGSV neoepitope ( A ) or CS-GAG stubs left following <t>chondroitinase</t> <t>ABC</t> treatment ( B ). The epitopes recognized by the antibodies are shown on the schematic of aggrecan. “0” condition refers to buffer control. In B , the red asterisk indicates a ubiquitous band. Numbering is according to bovine aggrecan, UniProt ID P16112-1. Domains are not drawn to scale. CF, cleavage fragment; CS, chondroitin sulfate; IGD, interglobular domain; KS, keratan sulfate-rich region.
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    Aggrecanase activity of ADAMTS9 MDTCS. Increasing concentrations of ADAMTS9 MDTCS (0.025–25 nM) or 5 nM full-length ADAMTS5 were incubated with bovine aggrecan (400 nM). Digests were stopped after two or 24 h and cleavage fragments deglycosylated and subjected to SDS-PAGE. Bands were detected using anti-aggrecan ARGSV neoepitope ( A ) or CS-GAG stubs left following <t>chondroitinase</t> <t>ABC</t> treatment ( B ). The epitopes recognized by the antibodies are shown on the schematic of aggrecan. “0” condition refers to buffer control. In B , the red asterisk indicates a ubiquitous band. Numbering is according to bovine aggrecan, UniProt ID P16112-1. Domains are not drawn to scale. CF, cleavage fragment; CS, chondroitin sulfate; IGD, interglobular domain; KS, keratan sulfate-rich region.
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    Image Search Results


    ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with chondroitinase ABC (chABC) at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.

    Journal: EMBO Molecular Medicine

    Article Title: A neuroprotective tetrapeptide for treatment of acute traumatic brain injury

    doi: 10.1038/s44321-025-00312-5

    Figure Lengend Snippet: ( A ) Fluorescence polarization (FP) measurement of CAQK binding to CSPG. FAM-CAQK (20 nM) was incubated with CSPG (200 nM) for 60 min at 37 °C. Binding with CSPG pretreated with chondroitinase ABC (chABC) at two concentrations low (5mU) and high (15mU) was compared to untreated CSPG. ( B ) FP assay to assess the binding of CAQK to brain ECM from different sources. FAM-labeled CAQK (20 nM) was incubated for 1 h at 37 °C with purified CSPG isolated from chicken brain (1 μM) or supernatant collected from cultured U251 human glioblastoma cell line. ( C ) Top hits from proteomic analysis of CSPG complex isolated from chicken brains and U251 conditioned media. ( D ) FP assay of binding of different peptides to TnC. FAM-labeled peptides (20 nM) were incubated with TnC (1 μM) for 1 h at 37 °C. ( E ) FP assay to assess the effect of the thiol group in cysteine of CAQK on binding to TnC. FAM-labeled CAQK (20 nM) was incubated with TnC (1 μM) for 1 h at 37 °C in the presence of GSH and Iodoacetamide. n = 3.

    Article Snippet: Chondroitinase ABC enzyme , AMS Bio , AMS.E1028-02.

    Techniques: Fluorescence, Binding Assay, Incubation, FP Assay, Labeling, Purification, Isolation, Cell Culture

    Aggrecanase activity of ADAMTS9 MDTCS. Increasing concentrations of ADAMTS9 MDTCS (0.025–25 nM) or 5 nM full-length ADAMTS5 were incubated with bovine aggrecan (400 nM). Digests were stopped after two or 24 h and cleavage fragments deglycosylated and subjected to SDS-PAGE. Bands were detected using anti-aggrecan ARGSV neoepitope ( A ) or CS-GAG stubs left following chondroitinase ABC treatment ( B ). The epitopes recognized by the antibodies are shown on the schematic of aggrecan. “0” condition refers to buffer control. In B , the red asterisk indicates a ubiquitous band. Numbering is according to bovine aggrecan, UniProt ID P16112-1. Domains are not drawn to scale. CF, cleavage fragment; CS, chondroitin sulfate; IGD, interglobular domain; KS, keratan sulfate-rich region.

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of ADAMTS9 proteoglycanase activity: Comparison with ADAMTS1, ADAMTS4, and ADAMTS5

    doi: 10.1016/j.jbc.2025.110301

    Figure Lengend Snippet: Aggrecanase activity of ADAMTS9 MDTCS. Increasing concentrations of ADAMTS9 MDTCS (0.025–25 nM) or 5 nM full-length ADAMTS5 were incubated with bovine aggrecan (400 nM). Digests were stopped after two or 24 h and cleavage fragments deglycosylated and subjected to SDS-PAGE. Bands were detected using anti-aggrecan ARGSV neoepitope ( A ) or CS-GAG stubs left following chondroitinase ABC treatment ( B ). The epitopes recognized by the antibodies are shown on the schematic of aggrecan. “0” condition refers to buffer control. In B , the red asterisk indicates a ubiquitous band. Numbering is according to bovine aggrecan, UniProt ID P16112-1. Domains are not drawn to scale. CF, cleavage fragment; CS, chondroitin sulfate; IGD, interglobular domain; KS, keratan sulfate-rich region.

    Article Snippet: Membranes were incubated overnight at 4 °C with the following antibodies, all prepared in 0.5% bovine serum albumin/PBS: rabbit polyclonal anti-Vc, recognizing the versican sequence 432 TVPKDPEAAEARRG 445 spanning the E441-A442 cleavage site in V1 GAGβ domain (1 μg/ml) ( ); rabbit polyclonal anti-DPEAAE neoepitope (Life Technologies, PA1-1748A, 2 μg/ml) which only detects versikine, the N-terminal versican fragment generated after proteolysis at E441-A442 in GAGβ; mouse monoclonal anti-ARGSV neoepitope (BC3) recognizing aggrecanase cleavage at E392-A393 (Life Technologies, MA3-16888, 4 μg/ml); mouse monoclonal 2B6, recognizing the CS stubs remaining on proteoglycans after chondroitinase ABC treatment of proteoglycans with chondroitinase ABC (AMS Biotechnology Europe, 270432-CS, 1:100); goat polyclonal anti-biglycan (Bio-Techne Ltd, AF2667, 0.4 μg/ml).

    Techniques: Activity Assay, Incubation, SDS Page, Control